Systematic Karyology and Embryology of Higher Plants

Head ao. Univ.-Prof. Dr. Johann Greilhuber

• Staff
• Research
• Teaching
• Selected References

Mag. Dr. Eva M. TEMSCH

Research

The primary focus of this laboratory is genome size research: the methodology of measurement, the variation of genome size and its biological significance, and the use of genome size data in plant systematics. Karyosystematic and cyto-embryological studies are also conducted.

Instruments available: Zeiss Axiophot epifluorescence microscope, several Zeiss Standard microscopes equipped with phase and Differential Interference Contrast optics, Cell Imaging and Retrieval System (CIRES) for image analysis and DNA content measurements, Partec CyFlow ML flow cytometer, MICRON high performance microtome, Reichert-Jung cryomicrotome.

Teaching

Lectures on "Fundamentals of karyosystematics", "Embryology and development (botany)", lab courses on karyosystematics, embryology, and contributions to the obligatory courses of the Institute.

Selected References

See also Lists of Publications of the Institute of Botany

Ricca M., Beecher F. W., Boles S. B., Temsch E. M., Greilhuber J., Shaw A. J. (2008)

Cytotype variation and allopolyploidy in North American species of the Sphagnum subsecundum complex. American Journal of Botany 95(12): 1606-1620.

Temsch E. M., Greilhuber J., Hammett K. R. W., Murray B. G (2008)

Genome size in Dahlia Cav. (Asteraceae – Coreopsideae). Plant Systematics and Evolution 276: 157-166.

Greilhuber J. (2008)

Cytochemistry and C-values: The less-well-known world of nuclear DNA amounts. Ann. Bot. 101: 791-804.

Loureiro J., Dolezel J., Greilhuber J., Santos C., Suda J. (2008)

Plant flow cytometry – far beyond the stone age. Cytometry Part A 73A: 579-580.

Hörandl E., Cosendai A. C., Temsch E. M. (2008)

Understanding the geographic distributions of apomictic plants: a case for a pluralistic apporach. Plant Ecology and Diversity 1(2): 309-320.

Dolezel J., Greilhuber J., Suda J. (2007)

Estimation of nuclear DNA content in plants using flow cytometry. Nature Protocols 2(9): 2233-2244.

Raycheva T., Temsch E. M., Dimitrova D. (2007)

Rumex pulcher (Polygonaceae) in the Bulgarian flora - distribution, morphology, and karyology. Phytologia Balcanica 13(3): 321-330.

Greilhuber J., Temsch E. M., Loureiro J. C. M. (2007)

Nuclear DNA content measurement. In: Flow Cytometry with Plant Cells (Dolezel J., Greilhuber J., Suda J., eds.). WILEY-VCH Verlag, Weinheim, pp. 67-101.

Dolezel J., Greilhuber J., Suda J. (2007)

Flow cytometry with plants: an overview. In: Flow Cytometry with Plant Cells (Dolezel J., Greilhuber J., Suda J., eds.). WILEY-VCH Verlag, Weinheim, pp. 41-65.

Dolezel J., Greilhuber J., Suda J. (eds.) (2007)

Flow Cytometry with Plant Cells. WILEY-VCH Verlag, Weinheim. 454 pp.

Greilhuber J., Borsch T., Müller K., Worberg A., Porembski S., Barthlott W. (2006)

Smallest angiosperm genomes found in Lentibulariaceae, with chromosomes of bacterial size. Plant Biology 8: 770-777.

Weiss-Schneeweiss H.,Greilhuber J., Schneeweiss G. (2006)

Genome size evolution in holoparasitic Orobanche (Orobanchaceae) and related genera. Amer J Bot 93: 148-156.

Bancheva S.,Greilhuber J. (2006)

Genome size in Bulgarian Centaurea s.l. (Asteraceae). Plant Syst Evol 257:95-117.

Obermayer R., Greilhuber J. (2006)

Cryptopolyploidy revisited: the case of Vinca (Apocynaceae). Plant Syst Evol 256: 201-208.

Paun O., Greilhuber J., Temsch E. M., Hörandl E. (2006)

Patterns, sources and ecological implications of clonal diversity in apomictic Ranunculus carpaticola (Ranunculus auricomus complex, Ranunculaceae) Molecular Ecology 15: 897-910.

Ebert I., Greilhuber J. (2005)

Developmental switch during embryo sac formation from a bisporic mode to the tetrasporic Fritillaria type in Hyacinthoides vincentina (Hoffmannsegg & Link) Rothm. (Hyacinthaceae). Acta Biol Cracov, Ser Bot 47: 179-184.

Obermayer R., Greilhuber J. (2005)

Does genome size in Dasypyrum villosum vary with fruit colour? Heredity 95: 91-95.

Aagaard S. M. D. Sastad S. M., Greilhuber J., Moen A. (2005)

A secondary hybrid zone between diploid Dactylorhiza incarnata ssp. cruenta and allotetraploid D. lapponica (Orchidaceae). Heredity 94: 488-496.

Greilhuber J., Dolezel J., Lysak M. A., Bennett M. D. (2005)

The origin, evolution and proposed stabilization of the terms ´Genome Size´ and ´C-value´ to describe nuclear DNA contents. Ann Bot 95: 255-260.

Greilhuber J. (2005)

Intraspecific variation in genome size in angiosperms: Identifying its existence. Ann Bot 95: 91-98.

Albach D., Greilhuber J. (2004)

Genome size variation and evolution in Veronica. Ann Bot 94: 897-911.

Greilhuber J., Leitch I. J., Bennett M.D. (2004)

Bryophyte DNA C-values database (release 2.0, Dec. 2004). http://www.kew.org/cval/homepage.html

Moscone E., Baranyi M., Ebert I., Greilhuber J., Ehrendorfer F., Hunziker A. T. (2003)

Analysis of nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and Feulgen densitometry. Ann Bot 92: 21-29.

Dolezel J., Bartos J., Voglmayr H., Greilhuber J. (2003)

Nuclear DNA content and genome size of trout and human. Cytometry 51A: 127-128.

Hörandl E., Greilhuber J. (2002)

Diploid and autotetraploid sexuals and their relationships to apomicts in the Ranunculus cassubicus group: Insights from DNA content and isozyme variation. Plant Syst Evol 234: 85-100.

Greilhuber J., Sastad S., Flatberg K I. (2003)

Ploidy determination in Sphagnum samples from Svalbard, Arctic Norway, by DNA image cytometry. J Bryol 25: 235-239.

Temsch E. M., Obermayer R., Dolezel J., Greilhuber J. (2001)

Application of an optical immersion-gel in a flow cytometer with horizontally oriented objective. Biotechnic and Histochemistry 76: 11-14.

Greilhuber J., Temsch E. M. (2001)

Feulgen densitometry: some observations relevant to best practice in quantitative nuclear DNA content determination. Acta Botanica Croatica 60: 285-298.

Vilhar B., Greilhuber J., Dolenc Koce, J., Temsch E.M., Dermastia M. (2001)

Plant genome size measurement with DNA image cytometry. Ann Bot 87: 719-728.

Temsch E.M., Greilhuber J. (2001)

Genome size in Arachis duranensis: a critical study. Genome 44: 826-830.

Dimitrova D., Greilhuber J. (2001)

C-banding patterns and quantitative karyotype characteristics of Bulgarian species of Crepis (Asteraceae) Plant Biol 3: 88-97.

Temsch E. M., Greilhuber J. (2000)

Genome size variation in Arachis hypogaea and A. monticola re-evaluated. Genome 43: 449-451.

Greilhuber J., Ebert I., Lorenz A., Vyskot B. (2000)

Origin of facultative heterochromatin in the endosperm of Gagea lutea (Liliaceae). Protoplasma 212: 217-226.

Dimitrova D., Ebert I., Greilhuber J., Kozhuharov S. (1999)

Karyotype constancy and genome size variation in Bulgarian Crepis foetida s. l. (Asteraceae). Plant Syst Evol 217: 245-257.

Obermayer R., Greilhuber J. (1999)

Genome size in Chinese soybean accessions - stable or variable? Ann Bot 84: 259-262.

Greilhuber J. (1998)

Intraspecific variation in genome size: A critical reassessment. Ann Bot 82, Suppl. A: 27-35.

Temsch E. M., Greilhuber J., Krisai R. (1998)

Genome size in Sphagnum (peat moss). Bot Acta 111: 325-330.

Voglmayr H., Greilhuber J. (1998)

Genome size determination in Peronosporales (Oomycota) by Feulgen image analysis. Fungal Genetics and Biology 25: 181-195.

Dolezel J., Greilhuber J., Lucretti S., Meister A., Lysak M. A., Nardi L., Obermayer R. (1998)

Plant genome size estimation by flow cytometry: Inter-laboratory comparison. Ann Bot 82, Suppl. A: 17-26.

Buzek J., Ebert I., Ruffini-Castiglione M., Siroky J., Vyskot B., Greilhuber J. (1998)

Structure and DNA methylation pattern of partially heterochromatinised endosperm nuclei in Gagea lutea (Liliaceae). Planta 204: 506-514.

Buzek J., Riha K., Siroky J., Ebert I., Greilhuber J., Vyskot B. (1998)

Histone H4 underacetylation in plant facultative heterochromatin. Biol Chem 379: 1235-1241.

Greilhuber J., Obermayer R. (1997)

Genome size variation in Cajanus cajan (Fabaceae): A reconsideration. Plant Syst Evol 212: 135-141.

Greilhuber J. (1997)

The problem of variable genome size in plants (with special reference to woody plants). In: Borzan Z., Schlarbaum S. E. (Eds.) Cytogenetic studies of forest trees and shrub species. pp. 13-34. Zagreb: Croatian Forests Inc., Faculty of Forestry, University of Zagreb.

Baranyi M., Greilhuber J. (1996)

Flow cytometric and Feulgen densitometric analysis of genome size variation in Pisum. Theor Appl Genet 92: 297-307.

Baranyi M., Greilhuber J. (1996)

Genome size in wild Pisum species. Theor Appl Genet 93: 717-721.

Greilhuber J., Ebert I. (1994)

Genome size variation in Pisum sativum. Genome 37: 646-655.

Berg C., Greilhuber J. (1992)

Cold-sensitive chromosome regions and their relation to constitutive heterochromatin in Cestrum parqui (Solanaceae). Genome 35: 921-930.